BP 303 T. PHARMACEUTICAL MICROBIOLOGY (Theory) || 3rd Semester || B Pharmacy || Unit-1 ||

BP 303 T. PHARMACEUTICAL MICROBIOLOGY (Theory)

 

Unit I

Introduction

·     History of microbiology, its branches, scope and its importance.

·      Introduction to Prokaryotes and Eukaryotes

·   Study of ultra-structure and morphological classification of bacteria,

·       Nutritional requirements,

·       Raw materials used for culture median physical parameters for growth,

·       Growth curve,

·       Isolation and preservation methods for pure cultures,

·       Cultivation of anaerobes,

·       Quantitative measurement of bacterial growth (total & viable count).

·       Study of different types of phase constrast microscopy,

·       Dark field microscopy and electron microscopy.

 

 Unit II

·       Identification of bacteria using staining techniques (simple, Gram’s &Acid fast staining) and biochemical tests (IMViC).

·       Study of principle, procedure, merits, demerits and applications of physical,

·       chemical gaseous, radiation and mechanical method of sterilization.

·       Evaluation of the efficiency of sterilization methods.

·       79 Equipments employed in large scale sterilization.

·       Sterility indicators.

 

 Unit III

·       Study of morphology,

·       classification,

·       reproduction/replication and cultivation of Fungi and Viruses.

·       Classification and mode of action of disinfectants Factors influencing disinfection,

·       antiseptics and their evaluation.

·       For bacteriostatic and bactericidal actions Evaluation of bactericidal & Bacteriostatic.

·       Sterility testing of products (solids, liquids, ophthalmic and other sterile products) according to IP, BP and USP.

 

 

 Unit IV

·       Designing of aseptic area, laminar flow equipments;

·       study of different sources of contamination in an aseptic area and methods of prevention,

·       clean area classification.

·       Principles and methods of different microbiological assay.

·       Methods for standardization of antibiotics,

·       vitamins and amino acids.

·       Assessment of a new antibiotic.

 

 

Unit V

·        Types of spoilage,

·       factors affecting the microbial spoilage of pharmaceutical products,

·       sources and types of microbial contaminants,

·       assessment of microbial contamination and spoilage.

·       Preservation of pharmaceutical products using antimicrobial agents,

·       evaluation of microbial stability of formulations.